By Bhushan, Vikas

ISBN-10: 1405104090

ISBN-13: 9781405104098

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Mol. Biol. 16, 212. Harfenist, Ε. , and Craig, L. C. (1952). / . Amer. Chem. Soc. 74, 3087. Harris, J. , and N a u g h t o n , M . A. (1956). Arch. Biochem. Biophys. 65, 427 Howell, S. , and Lacey, P. E . (1969). J. Cell Biol. 4 2 , 695. Inada, Y . (1961). / . Biochem. {Tokyo) 49, 217. Katsoyannis, P. G. (1969). , Intern. Cong. Ser. 172, 379. , Leach, S. , and Scheraga, Η. Α. (1960). / . Amer. Chem. Soc. 8 2 , 571. Lindsay, D . , and Shall, S. (1971). Biochem. J. 221 (in press). Low, B . , and Einstein, J.

T. L. B l u n d e l l : If the concentration of insulin in the blood is 10"^^ Μ [S. A. Berson and R. S. Yalow, Amer. J. Med. 4 0 , 676 (1966)] and the association constant of the dimer is 10^ [J. A. Rupley, R. D . Renthal, and M . Praissman, Biochem. Biophys. Acta 140, 185 (1967)], then the insulin will exist mainly as monomers; thus, the most likely active form is the THE STRUCTURE OF INSULIN 35 monomer. However, the quoted association constant was obtained at p H 2, and the value must be modified to allow for the different charge distribution at physiological p H .

T. L. B l u n d e l l : I have indeed been very selective in choosing a hexagonal /3-granule of a rat to compare with tk^ rhombohedral crystals. N o t only are there different shapes /3-granules in the rat pancreas, but also there appear to be m a n y different periodicities and shapes exemplified by /3-gran^les of other species. [T. Sato, L. Herman, and P. J. Fitzgerald, J. Gen. Comp. Endocrinol. 7, 132 (1966)]. In rat granules some of the differences of appearance m a y result from the angle at which the granules are viewed in the electron micrograph, but they m a y also result from different types of crystal packing.

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Anatomy by Bhushan, Vikas

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