By Walker J.M. (ed.), Pollard J.W. (ed.)

CIP cites the sequence because the major access. Covers equipment for growing mammalian fibroblastic mobile cultures and preserving tradition stipulations for epithelial, neuronal, and hematopoietic cells between others. realization is given to the range of tradition media and extracellular matrices had to hold the differential services of the aesthetic cells. additionally describes tradition strategies for either dwelling and glued cells.

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This speed depends upon the individual type of culture reactor. Stir at a speed sufficient to keep the cells in homogeneous suspension. Do not use speeds that allow cells to settle out at the bottom of the culture, 5. Monitor cell growth at least daily by taking a small sample, either through a special sampling device or removing the vessel to a laminar flow cabinet and using a pipet, and carry out a viable cell count (Trypan blue stain and a hemocytometer). 6. , all ports stoppered with no filters), then the pH will fall.

This will keep for 1 wk at 4°C. 2. B Cell Growth Factor (BCGF). 3. Clostridium perfringens neuraminidase. 4. EBV suspension, 5 x lo4 Transforming Units (TFU). 5. F(ab’), fragment of goat anti-human IgM (p-chain specific). 6. Ficoll-paque. 7. RPM1 growth medium contains 5,10, or 15% fetal calf serum, 2 mM Lglutamine, 100 pg/mL penicillin, and 100 mg/mL streptomycin. 8. Growth medium containing 10% FCS, and 2-Mercaptoethanol (2 x lo4 M). 9. Hanks’ balanced salt solution pH 4 (seeAppendix). 10. Helix pomatia Lectin-Sepharose 6MB.

L to lOOliter of aunit processsystembased on 3 mm diameter glass spheres for the production of four strains of FMDV from BHK monolayer cells. 23,551-565. Chapter Mycoplasma 7 Detection J. M. Mowles 1. Introduction Mycoplasma is the generic term used by cell biologists to denote organisms belonging to the Order Mycoplasmatales, which can infect cell cultures. Of particular interest are those organisms that belong to the Families Mycoplasmataceae (Mycoplasma) and Acholeplasmataceae (Acholeplasma).

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Animal Cell Culture by Walker J.M. (ed.), Pollard J.W. (ed.)


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